RFLP simulation

Find the criminal using the RFLP method.

DNA found in the crime scene

CTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCTTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTT

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Three suspects

Suspect A

CTACTCCTGCTCGCATCTGCTATAGTGGAGACCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCTTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTTGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTT

Suspect B

CTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCTTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTT

Suspect C

CTACTCCTGCTCGCATCTGCTATAGTGGAGACCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCTTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGTTCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTTGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTT

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Mission

1. Perform the RFLP analysis using two restriction enzymes, HaeIII and BfaI, in the NEBcutter website.
2. Find out the criminal (suspect A, B, or C?) by comparing their RFLP patterns with that of DNA found in the crime scene.

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Protocol

DNA found in the crime scene is used as an example. First, paste the sequence and submit.

This gives you the list of restriction enzymes that cut the DNA. Because we want to know the RFLP pattern of two restriction enzymes, HaeIII and DpnI, click "custom digest."

Now you can see the list of restriction enzymes that cut this DNA fragment. Check HaeIII and BfaI and click "Digest."

Here is your digestion pattern.

By clicking "View gel," you can see the agarose gel electrophoresis pattern of the digested DNA fragments. Because the DNA fragments are relatively small (around 100 bp or less), you need to change the gel type to clearly separate them.